Identification of the virulence factors and suppressors of. Towards a common library and tools for web molecular graphics molvaeurovis proceedings. Cryoem structure of ageratum yellow vein virus ayvv select a different viewer. Identification and characterization of citrus yellow vein. Association of an alphasatellite with tomato yellow leaf. These viruses are spreading rapidly in a devastating manner throughout different crops and weed species in various parts of the world. Ageratum leaf curl cameroon virus alccmv ageratum yellow vein hualian virus ayvhuv allamanda leaf curl virus allcv allamanda leaf mottle distortion virus allmdv alternanthera yellow vein virus alyvv asystasia begomovirus 2 abgv2 bean calico mosaic virus bcamv bean chlorosis virus bclv bean chlorotic mosaic virus bchmv. Geminiviruses have singlestranded dna circular genomes and infect various crops such as tomato and pepper worldwide. Molecular characterization of tomato leaf curl palampur. As a member of the wwpdb, the rcsb pdb curates and annotates pdb data according to agreed upon standards. Using the rdp3 recombination detection program, a recombination event involving croton yellow vein mosaic virus india. Ageratum yellow vein china virus is a distinct begomovirus.
Abstract molecular features and genomic organization were determined for citrus yellow vein clearing virus cyvcv, the putative viral causal agent of yellow vein clearing disease of lemon trees, reported in pakistan, india, and more recently in turkey and china. The purpose of this video is to inform farmers, producers and other tomato growers about guams ageratum yellow vein virus ayvv. Degenerate primers, pa and pb, were designed to amplify part of the intergenic region and coat protein cp gene of dna. Ageratum yellow vein virus isolated from tomato plants with.
Symptoms of ageratum yellow vein virus ayvv guam on tomato. Association of an alphasatellite with tomato yellow leaf curl. Taxonomy navigation begomovirus all lower taxonomy nodes 12 common name isynonym iother names. First report of malvastrum yellow vein virus infecting. Apr 28, 2010 dna sequence analyses of the pcr products revealed that the symptomatic tomato plants were associated with ageratum yellow vein virus ayvv infection. Epidemiology of okra yellow vein mosaic virus oyvmv and its management through tracer, mycotal and imidacloprid 1744. Whiteflytransmitted geminiviruses family geminiviridae, genus begomovirus cause severe disease epidemics of tomato and pepper in indonesia. Genome organization of ageratum yellow vein virus, a. A tomato virus severity scale figure 4 was created and used to visually evaluate the tomato varieties for ageratum yellow vein virus ayvv. S1 in the supplemental material, and total nucleic acids were extracted from leaf tissue as described previously. Tobacco curly shoot virus tbcsv, tobacco leaf curl yunnan virus tblcynv, tomato yellow leaf curl china virus tylccnv, tomato yellow leaf curl thailand virus tylcthv, malvastrum yellow vein virus myvv, malvastrum yellow vein yunnan.
Temporal distribution and pathogenicity of the predominant. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. A begomovirus associated with ageratum yellow vein disease in. We were able to identify a third begomovirustomato leaf curl palampur virus tolcpmv, from pumpkin showing typical symptoms of the disease at. Pdf genome organization of ageratum yellow vein virus, a. Several examples of the defective dna have been cloned from purified virus specific supercoiled dna and characterized by sequence analysis. First report of ageratum yellow vein virus infecting tomato. The majority of these isolates were found to be infected with 1 or 2 of the following 7 viruses. Alphasatellites are single stranded satellite dna that are dependent on a virus for transmission. Ageratum enation virusa begomovirus of weeds with the. Previous studies have found that the diversity of begomovirusassociated dna. Polymerase chain reaction detection and sequence analysis showed that samples collected from yunnan were mainly infected by tobacco curly shoot virus tbcsv associated with ageratum. Begomovirus associated with yellow vein mosaic disease of. Soybean crinkle leaf virus as of 2015 after ictv references.
Ageratum yellow vein virus isolated from tomato plants with leaf curl. A virus isolate, y206, was obtained from ageratum conyzoides showing yellow vein symptoms from baoshan in the yunnan province of china. By contrast, other betasatellitedependent helper viruses, including tomato yellow leaf curl china virus, cotton leaf curl multan virus and ageratum yellow vein virus would not prevail in the absence of functional betasatellite. Genome organization of ageratum yellow vein virus, a monopartite whiteflytransmitted geminivirus isolated from a common weed. Ageratum yellow vein virus ayvv, tomato leaf curl java virus tolcjv, tomato yellow leaf curl indonesia virus tylcidv, and pepper yellow leaf curl. The begomovirus was identified as an isolate of ageratum yellow vein china virus ayvcnv with 91% nucleotide sequence identity to ayvcnvp157 eu487045, while the alphasatellite molecule was. In neighborjoining tree, the coat protein of mimosa yellow vein virus adw83735 place in monophyletic clusters of 74 bootstrap values with av1 protein of ageratum yellow vein china virus cad90081. A new alphasatellite molecule associated with ageratum yellow. Ayvtv stands for ageratum yellow vein taiwan virus. Ayvhuv was detected occasionally in 2003 and tolchsv only in. Tomato leaf curl new delhi virus and squash leaf curl china virus are known to be associated with this disease in india.
Yellow vein disease of ageratum conyzoides, a weed species that is widely distributed throughout asia, has been attributed to infection by the geminivirus ageratum yellow vein virus ayvv. Jun 18, 2018 the structure of ageratum yellow vein virus. A specific 500 bp product was consistently amplified from total dna extracts from symp. Association of an alphasatellite with tomatoyellowleafcurlvirus and ageratumyellowveinvirus in japan is suggestive of a recent introduction. Characterization of alphasatellites associated with. Pdf a fulllength copy of a single genomic component of the whitefly transmitted geminivirus ageratum yellow vein virus ayvv has been. Jan 14, 2014 a number of monopartite begomoviruses have been identified in japan. A number of monopartite begomoviruses have been identified in japan. These include tobacco leaf curl japan virus tblcjv and honeysuckle yellow vein mosaic virus hyvmv, which cause tomato yellow dwarf disease 16,17, and ageratum yellow vein virus ayvv, which has so far only been reported from okinawa and causes problems in tomato there. No significant recombination event was detected in the betasatellite sequence.
Summary samples of emilia sonchifolialeaves showing con spicuous yellow veins were collected in the chinese province of fujian. Ageratum yellow vein india betasatellite wikispecies. To identify possible begomoviruses, total dna was extracted from the leaf sample. Seven monopartite begomoviruses have been identified causing tlcd in china. Abstract ageratum conyzoides plants exhibiting yellow vein symptoms, collected near haikou, hainan province.
A new alphasatellite molecule associated with ageratum. Geminiviruses cause diverse disease symptoms such as mosaic, chlorosis, necrosis, malformation, and stunting andou et al. Most begomoviruses have bipartite genomes dnas a and b, but only a dna a has been identified. The bnyvv is a member of the genus benyvirus and is responsible for rhizomania, a disease of sugar beet rhizo. Ayvtv is defined as ageratum yellow vein taiwan virus rarely. Ageratum yellow vein virus is a plant pathogenic virus of the family geminiviridae. Etiology of ageratum yellow vein diseases in south china. Phylogenetics and in silico docking studies between coat.
These include tobacco leaf curl japan virus tblcjv and honeysuckle yellow vein mosaic virus hyvmv, which cause tomato yellow dwarf disease 16,17, and ageratum yellow vein virus ayvv, which has so far only been reported from okinawa and causes problems in tomato. Soybean mosaic virus, a virus sometimes referred to as crinkle. Tobacco leaf curl disease tlcd is caused by begomoviruses in geminiviridae, and infected plants exhibit leaf thickening, downward leaf curling, vein swelling as well as stunting symptoms. A unique virus complex causes ageratum yellow vein disease ncbi. The first alphasatellites were described in 1999 and were associated with cotton leaf curl disease and ageratum yellow vein disease. These molecules are visualized, downloaded, and analyzed by users who range from students. The rcsb pdb also provides a variety of tools and resources. Jul 17, 2014 here, we constructed a twot dna vector carrying a hairpin of the intergenic region igr of ageratum yellow vein virus ayvv, residing in an intron inserted in an untranslatable nucleocapsid. Association of an alphasatellite with tomato yellow leaf curl virus and ageratum yellow vein virus in japan is suggestive of a recent introduction by muhammad shafiq shahid 1,2, masato ikegami 1, abdul waheed 2, rob w. Also excluded are common names and obsolete names for viruses for a list of virus genera, see list of virus genera for a list of virus families and subfamilies, see list of. Ayvv is defined as ageratum yellow vein virus somewhat frequently.
Identification of a new begomovirus associated with yellow. In this study, we demonstrated that the core nucleotides in the putative. A unique virus complex causes ageratum yellow vein disease. Ukbased biologists have unveiled the complex 3d structure of one of the worlds most lethal families of plant viruses using singleparticle cryoem, researchers at the astbury centre for structural molecular biology, university of leeds, resolved the structure of the. Sequences were assembled and analyzed with the aid of the lasergene package of sequence analysis software dnastar inc. This is a list of all virus species, including satellites and viroids. Journal of plant pathology 2006, 88 1, 8388 edizioni ets pisa, 2006 83 molecular characterization of begomoviruses infecting ludwigia hyssopifolia j. Sequence analysis by using blastn revealed the highest 98% nucleotide sequence identities with ageratum yellow vein china virus aj558120, 97% with ageratum yellow vein virus g129 am9407 also 97% with ageratum yellow vein china virus g68 aj849916 and 95% with ageratum yellow vein. A nanoviruslike dna component associated with yellow vein. Novel defective interfering dnas associated with ageratum. Characterization of the cryptic av3 promoter of ageratum. Molecular identification of tobacco leaf curl disease in. Beet necrotic yellow vein virus bnyvv is a plant virus, transmitted by the plasmodiophorid polymyxa betae. Among the assembled contigs, one single contig 2570 nt assembled from 312 reads from one library was matched to ageratum yellow vein virus ayvv, kc172826.
The density is segmented and colored to highlight the 11 unique copies of the ayvv cp in the d5 asymmetric. Ageratum yellow vein virus isolated from tomato plants. Ageratum yellow vein virus begomovirus tomato vietnam. Citation images created using mol should cite the pdb id, the corresponding structure publication, mol d. The aim of present work was to understand the functions of v2 and. Request pdf ageratum yellow vein virus isolated from tomato plants with leaf curl on ishigaki island, okinawa, japan in 2005, severe leaf curling and. Three times throughout the wet season trial, fields were evaluated for ayvv, approximately one, two, and three months after transplant. Four tomatoinfecting begomoviruses have been reported from java island.
These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists. Ageratum yellow vein taiwan virus how is ageratum yellow. The genome is a single circular single strand dna molecule. Solanum lycopersicum, tomato yellow leaf curl virus, ageratum yellow vein virus. Ageratum conyzoides is a common weed in agricultural regions in asia. Virus sources, dna extraction, and virus detection. The pcr product was cloned, partially sequenced and submitted in ncbi genbank accession hq876467. Ageratum yellow vein virus, papaya leaf curl china virus, and an unidentified. Here we report the structure of ageratum yellow vein virus at 3. May 12, 2011 using the rdp3 recombination detection program, a recombination event involving croton yellow vein mosaic virus. In this study, we have cloned and sequenced 20 different isolates of dna.
Defective dna forms of the geminivirus ageratum yellow vein virus ayvv have been identified in naturally infectedageratum conyzoidesplants. Epidemiology of okra yellow vein mosaic virus oyvmv and. Chilli leaf curl palampur virus is a distinct begomovirus. Jun 18, 2018 structure of geminivirus, ageratum yellow vein virus university of leeds. Like tomato yellow leaf curl virus and tlcv, ayvv dna a is infectious in tomato and nicotiana spp. Using the rdp3 recombination detection program, a recombination event involving croton yellow vein mosaic virus.
In addition to a single ayvv genomic component dna a, we have previously demonstrated that infected plants contain chimeric defective viral components, comprising dna a and nongeminiviral sequences, that act as defective interfering dnas. Viruses free fulltext a naturally occurring defective. Wisconsin genetics computer group software devereux et al. This definition appears somewhat frequently and is found in the following acronym finder categories. Here, we constructed a twot dna vector carrying a hairpin of the intergenic region igr of ageratum yellow vein virus ayvv, residing. The team studied a type of geminvirus called ageratum yellow vein virus, which was produced in tobacco plants under carefully controlled conditions by researchers at the john innes centre in norwich. Ageratum yellow vein china virus is a distinct begomovirus species associated with a dnabeta molecule. Devastating plant virus is revealed in atomic detail. However, it has not shown whether v2, or c4 proteins encoded by tomato yellow leaf curl china virusboshan1 china. Excluded are other ranks, and other noncellular life such as prions. Pumpkin cultivation in india is affected by severe incidence of a yellow vein mosaic disease. A cryptic prokaryotic promoter, designated av3 promoter, has been previously identified in certain begomovirus genus, including ageratum yellow vein virus isolate nt ayvvnt. Zhou 1 institute of biotechnology, zhejiang university, hangzhou, 310029, p. A begomovirus associated with ageratum yellow vein disease in indonesia.
Phylogenetic relationships of the virus isolate genbank accession eu439259 at nucleotide level with ageratum yellow vein virus, ageratum enation virus, tobacco curly shoot virus and other begomovirus isolates reported from india and abroad. Dna sequence analyses of the pcr products revealed that the symptomatic tomato plants were associated with ageratum yellow vein virus ayvv infection. We confirmed ayvv transmission from the naturally infected weed host, ageratum conyzoides, to healthy tomato plants by the insect vector bemisia tabaci b biotype. It is one of the economically important diseases in tropical and subtropical tobaccogrowing areas. Aylcb clones have also previously been shown to be infectious in the presence of ageratum yellow vein virus, a virus that is not reported to be associated with this species of betasatellite, inducing typical yellow vein symptoms in a. Samples were collected in 2011 from tomato plants exhibiting typical tomato leaf curl disease symptoms in the vicinity of komae, japan. How is ageratum yellow vein taiwan virus abbreviated. Sequence analysis by using blastn revealed the highest 98% nucleotide sequence identities with ageratum yellow vein china virus aj558120, 97% with ageratum yellow vein virusg129 am9407 also 97% with ageratum yellow vein china virusg68 aj849916 and 95%. Satellites and other virusdependent nucleic acids group. Using the rdp3 recombination detection program, a recombination event involving croton yellow vein mosaic virusindia. Molecular variation of satellite dna beta molecules. Sequence comparisons revealed that the three isolates were most closely related to those of euphorbia leaf curl virus elcv, aj558121, ageratum yellow vein virus ayvv, fj869908 and papaya leaf curl guangdong virus palcugdv, fj869907, with 92. Pcr mediated amplification, cloning and sequencing of all begomovirus components from two plants from different fields showed the plants to be infected by tomatoyellowleafcurlvirus tylcv and ageratumyellowveinvirus ayvv. International committee on the taxonomy of viruses.
Cyvcv isolate y1 from adana, turkey, was used for deep sequencing analysis of the virusinduced small rna. Three times throughout the wet season trial, fields were evaluated for ayvv, approximately. Ayvtv ageratum yellow vein taiwan virus acronymfinder. Genome organization of ageratum yellow vein virus, a monopartite whiteflytransmitted geminivirus isolated from a common weed priscilla h.
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